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Please use this identifier to cite or link to this item: http://hdl.handle.net/10481/29165

Title: The immunomodulator PSK induces in vitro cytotoxic activity in tumour cell lines via arrest of cell cycle and induction of apoptosis
Authors: Jiménez-Medina, Eva
Berruguilla, Enrique
Romero García, Irene
Algarra López, Ignacio
Collado, Antonia
Garrido Torres-Puchol, Federico
García Lora, Ángel
Issue Date: 2008
Abstract: Background Protein-bound polysaccharide (PSK) is derived from the CM-101 strain of the fungus Coriolus versicolor and has shown anticancer activity in vitro and in in vivo experimental models and human cancers. Several randomized clinical trials have demonstrated that PSK has great potential in adjuvant cancer therapy, with positive results in the adjuvant treatment of gastric, esophageal, colorectal, breast and lung cancers. These studies have suggested the efficacy of PSK as an immunomodulator of biological responses. The precise molecular mechanisms responsible for its biological activity have yet to be fully elucidated.
Methods The in vitro cytotoxic anti-tumour activity of PSK has been evaluated in various tumour cell lines derived from leukaemias, melanomas, fibrosarcomas and cervix, lung, pancreas and gastric cancers. Tumour cell proliferation in vitro was measured by BrdU incorporation and viable cell count. Effect of PSK on human peripheral blood lymphocyte (PBL) proliferation in vitro was also analyzed. Studies of cell cycle and apoptosis were performed in PSK-treated cells.
Results PSK showed in vitro inhibition of tumour cell proliferation as measured by BrdU incorporation and viable cell count. The inhibition ranged from 22 to 84%. Inhibition mechanisms were identified as cell cycle arrest, with cell accumulation in G0/G1 phase and increase in apoptosis and caspase-3 expression. These results indicate that PSK has a direct cytotoxic activity in vitro, inhibiting tumour cell proliferation. In contrast, PSK shows a synergistic effect with IL-2 that increases PBL proliferation.
Conclusion These results indicate that PSK has cytotoxic activity in vitro on tumour cell lines. This new cytotoxic activity of PSK on tumour cells is independent of its previously described immunomodulatory activity on NK cells.
Sponsorship: AGL was supported by FIS Postdoctoral Research Contract CP03/00111. Studies were partially supported by a grant from Kureha Chemical Industry (Japan).
Publisher: Biomed Central
Keywords: Apoptosis
Cell cycle
Drug screening assays
Fungal proteins
HeLa cells
Immunologic factors
Jurkat cells
Lymphocytes
Melanoma
Polysaccharides
Proteoglycans
URI: http://hdl.handle.net/10481/29165
ISSN: 1471-2407
Rights : Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License
Citation: Jiménez-Medina, E.; et al. The immunomodulator PSK induces in vitro cytotoxic activity in tumour cell lines via arrest of cell cycle and induction of apoptosis. BMC Cancer, 8: 78 (2008). [http://hdl.handle.net/10481/29165]
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