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Please use this identifier to cite or link to this item: http://hdl.handle.net/10481/28498

Title: A Portable Luminometer with a Disposable Electrochemiluminescent Biosensor for Lactate Determination
Authors: Martínez Olmos, Antonio
Ballesta Claver, Julio
Palma López, Alberto José
Valencia Mirón, María del Carmen
Capitán Vallvey, Luis Fermín
Issue Date: 2009
Abstract: A hand-held luminometer for measuring electrochemiluminescence (ECL) for lactate determination and based on one-shot biosensors fabricated using screen-printed electrodes is described. The lactate recognition system is based on lactate oxidase and the transduction system consists of electro-oxidation of luminol, with all the reagents immobilized in a Methocel membrane. The membrane composition and reaction conditions have been optimized to obtain adequate sensitivity. The luminometer is based on a large silicon photodiode as detector and includes a programmable potentiostat to initialize the chemical reaction and signal processing circuitry, designed to acquire a low level photocurrent with offset cancelation, low pass filtering for noise attenuation and adjustable gain up to 1012 V/A. The one-shot biosensor responds to lactate rapidly, with an acquisition time of 2.5 min, obtaining a linear dependence from 8 × 10−6 to 2 × 10−4 M, a detection limit of 2.4 × 10−6 M and a sensor-to-sensor reproducibility (relative standard deviation, RSD) of around 7–10 % at the medium level of the range.
Sponsorship: We acknowledge financial support from Ministerio de Educación y Ciencia, Dirección General de Enseñanza Superior (Spain) (Projects CTQ2005-09060-CO2-01, CTQ2005-09060-CO2-02) and from Junta de Andalucía (Projects P06-FQM-01467 and P08-FQM-3535).
Publisher: MDPI
Keywords: Portable instrument
Electrochemiluminescence measurement
Disposable biosensor
Lactate determination
URI: http://hdl.handle.net/10481/28498
ISSN: 1424-8220
Rights : Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License
Citation: Martínez Olmos, A.; et al. A Portable Luminometer with a Disposable Electrochemiluminescent Biosensor for Lactate Determination. Sensors, 9(10): 7694-7710 (2009). [http://hdl.handle.net/10481/28498]
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