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Please use this identifier to cite or link to this item: http://hdl.handle.net/10481/28494

Title: Novel Drug Delivery System Based on Docetaxel-Loaded Nanocapsules as a Therapeutic Strategy Against Breast Cancer Cells
Authors: Sánchez-Moreno, Paola
Boulaiz, Houria
Ortega Vinuesa, Juan Luis
Peula-García, José Manuel
Aránega Jiménez, Antonia
Issue Date: 2012
Abstract: In the field of cancer therapy, lipid nanocapsules based on a core-shell structure are promising vehicles for the delivery of hydrophobic drugs such as docetaxel. The main aim of this work was to evaluate whether docetaxel-loaded lipid nanocapsules improved the anti-tumor effect of free docetaxel in breast cancer cells. Three docetaxel-loaded lipid nanocapsules were synthesized by solvent displacement method. Cytotoxic assays were evaluated in breast carcinoma (MCF-7) cells treated by the sulforhodamine B colorimetric method. Cell cycle was studied by flow cytometry and Annexin V-FITC, and apoptosis was evaluated by using propidium iodide assays. The anti-proliferative effect of docetaxel appeared much earlier when the drug was encapsulated in lipid nanoparticles than when it was free. Docetaxel-loaded lipid nanocapsules significantly enhanced the decrease in IC50 rate, and the treated cells evidenced apoptosis and a premature progression of the cell cycle from G(1) to G(2)-M phase. The chemotherapeutic effect of free docetaxel on breast cancer cells is improved by its encapsulation in lipid nanocapsules. This approach has the potential to overcome some major limitations of conventional chemotherapy and may be a promising strategy for future applications in breast cancer therapy.
Sponsorship: The study was supported in part by grants from the Science and Innovation Ministry through the project REF: SAF2009-12295 and MAT2010-20370 (European FEDER support included), the “Instituto de Salud Carlos III” (Fondo de Investigación Sanitaria project No. P07-FQM03099) and the “Consejería de Innovación, Ciencia y Tecnología de la Junta de Andalucía” (Spain), by projects of excellence P07-FQM-2496 and P07-FQM03099 and P10-CTS-6270.
Publisher: MDPI
Keywords: Lipid nanoparticles
Docetaxel
Drug delivery
Breast cancer
URI: http://hdl.handle.net/10481/28494
ISSN: 1422-0067
1661-6596
Rights : Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License
Citation: Sánchez-Moreno, P.; et al. Novel Drug Delivery System Based on Docetaxel-Loaded Nanocapsules as a Therapeutic Strategy Against Breast Cancer Cells. International Journal of Molecular Sciences, 13(4): 4906-4919 (2012). [http://hdl.handle.net/10481/28494]
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