Universidad de Granada Digibug
 

Repositorio Institucional de la Universidad de Granada >
2.-Revistas >
ARS Pharmaceutica >
Ars Pharma 2010, Vol. 51 >
APh, vol. 51(3) >

Please use this identifier to cite or link to this item: http://hdl.handle.net/10481/27421

Title: Development and validation of spectrophotometric and ion pair chromatographic techniques for estimation of telmisartan and hydrochlorothiazide
Authors: Bhatia, N.M.
Shinde, H.V.
Bhatia, M.S.
Choudhari, P.B.
Ingale, K.B.
Issue Date: 2010
Abstract: Ultraviolet spectrophotometric and ion pair chromatographic methods have been developed for simultaneous estimation of telmisartan and hydrochlrothiazide from their tablet dosage form. The first method involves multiwavelength spectrophotometric estimation (Method 1) where interference due to hydrochlrothiazide at 286 nm (wavelength for estimation of telmisartan) was eliminated by recording absorbance difference at 286 nm and 308 nm whereas interference of telmisartan at 262 nm (wavelength for estimation of hydrochlrothiazide) was removed by recording absorbance difference at 262 nm and 282 nm. Linearity of the response was demonstrated by telmisartan in the concentration range of 5-35 μg/ml with a square correlation coefficient (r2) of 0.9995. Linearity of the response was demonstrated by hydrochlrothiazide in the concentration range of 3-21 μg/ml with a square correlation coefficient (r2) of 0.9992. The second method utilizes ion pair chromatography (Method 2) on a HIQ sil ODS column (250 mm length x 4.6 mm internal diameter) using methanol: 0.0025 M orthophosphoric acid (70:30 by volume pH 4.6) containing 0.1% 1-hexane sulphonic acid monohydrate sodium salt as mobile phase with UV detection at 259 nm over a concentration range of 20-120 μg/ml for telmisartan and 12.5-75 μg/ml for hydrochlrothiazide. Losartan potassium was used as the internal standard. Both the methods were applied successfully for the analysis of the two drugs from their tablet dosage form. The results of analysis were validated statistically and as per ICHQ2B guidelines. The developed methods are simple, selective and reproducible and can be applied for routine analysis of formulations containing telmisartan and hydrochlrothiazide.
Publisher: Universidad de Granada, Facultad de Farmacia
Keywords: Hydrochlorothiazide
Losartan Potassium
Multiwavelength Method
RP-HPLC
Telmisartan
Validation
URI: http://hdl.handle.net/10481/27421
ISSN: 0004-2927
Rights : Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License
Citation: Bhatia, N.M.; et al. Development and validation of spectrophotometric and ion pair chromatographic techniques for estimation of telmisartan and hydrochlorothiazide. Ars Pharm 2010; 51(3): 145-154. [http://hdl.handle.net/10481/27421]
Appears in Collections:APh, vol. 51(3)

Files in This Item:

File Description SizeFormat
Ars Pharm 2010;51(3)145-154.pdf160.11 kBAdobe PDFView/Open
Recommend this item

This item is licensed under a Creative Commons License
Creative Commons

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

 

Valid XHTML 1.0! OpenAire compliant DSpace Software Copyright © 2002-2007 MIT and Hewlett-Packard - Feedback

© Universidad de Granada