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Please use this identifier to cite or link to this item: http://hdl.handle.net/10481/24990

Title: Comparison of shor-term estrogenicity tests for identification of hormone-disrupting chemicals
Authors: Andersen, Helle Raun
Andersson, Anna-Maria
Arnold, Steven F.
Autrup, Herman
Barfoed, Marianne
Beresford, Nicola
Bjerregaard, Poul
Christiansen, Lisette
Gissel, Birgitte
Hummel, René
Jørgensen, Eva B.
Korsgaard, Bodil
Guevel, Remy Le
Leffers, Henrik
McLachlan, John A.
Møller, Anette
Nielsen, Jesper Bo
Olea, Nicolás
Oles-Karasko, Anita
Pakdel, Farzad
Pedersen, Knud L.
Pérez, Pilar
Skakkebæk, Niels
Sonnenschein, Carlos
Soto, Ana M.
Sumpter, John P.
Thorpe, Susan
Grandjean, Philippe
Issue Date: 1999
Abstract: The aim of this study was to compare results obtained by eight different short-term assays of estrogenlike actions of chemicals conducted in 10 different laboratories in five countries. Twenty chemicals were selected to represent direct-acting estrogens, compounds with estrogenic metabolites, estrogenic antagonists, and a known cytotoxic agent. Also included in the test panel were 17β-estradiol as a positive control and ethanol as solvent control. The test compounds were coded before distribution. Test methods included direct binding to the estrogen receptor (ER), proliferation of MCF-7 cells, transient reporter gene expression in MCF-7 cells, reporter gene expression in yeast strains stably transfected with the human ER and an estrogen-responsive reporter gene, and vitellogenin production in juvenile rainbow trout. 17β-Estradiol, 17α-ethynyl estradiol, and diethylstilbestrol induced a strong estrogenic response in all test systems. Colchicine caused cytotoxicity only. Bisphenol A induced an estrogenic response in all assays. The results obtained for the remaining test compounds—tamoxifen, ICI 182.780, testosterone, bisphenol A dimethacrylate, 4-n-octylphenol, 4-n-nonylphenol, nonylphenol dodecylethoxylate, butylbenzylphthalate, dibutylphthalate, methoxychlor, o,p′-DDT, p,p′-DDE, endosulfan, chlomequat chloride, and ethanol—varied among the assays. The results demonstrate that careful standardization is necessary to obtain a reasonable degree of reproducibility. Also, similar methods vary in their sensitivity to estrogenic compounds. Thus, short-term tests are useful for screening purposes, but the methods must be further validated by additional interlaboratory and interassay comparisons to document the reliability of the methods.
Sponsorship: This study was supported by grants from the European Commission (Biomedicine and Health Research and Technological Programme, BMH4-CT96-03 14), the Danish Environmental Research Programme (96.01.015.16), and the Danish Medical Research Council (9401656).
Publisher: National Institute of Environmental Health Sciences
Keywords: Estrogenic chemicals
Estrogens
Antiestrogens
Estrogenicity tests
Binding assay
Yeast
MCF-7
Vitellogenin
URI: http://hdl.handle.net/10481/24990
ISSN: 0091-6765
1552-9924
Rights : Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License
Citation: Andersen, H.R.; et al. Comparison of shor-term estrogenicity tests for identification of hormone-disrupting chemicals. Environmental Health Perspectives, 107(sup.1): 89-108 (1999). [http://hdl.handle.net/10481/24990]
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