Departamento de Fisiología Vegetalhttps://hdl.handle.net/10481/311902024-03-29T11:23:08Z2024-03-29T11:23:08ZNitrogen and photorespiration pathways, salt stress genotypic tolerance effects in tomato plants (Solanum lycopersicum L.)de la Torre González, AlejandroNavarro León, EloyBlasco León, BegoñaRuiz Sáez, Juan Manuelhttps://hdl.handle.net/10481/897532024-03-04T09:04:22ZNitrogen and photorespiration pathways, salt stress genotypic tolerance effects in tomato plants (Solanum lycopersicum L.)
de la Torre González, Alejandro; Navarro León, Eloy; Blasco León, Begoña; Ruiz Sáez, Juan Manuel
Nitrogen is necessary to synthesize compounds such as chlorophyll, amino acids, nucleic acids, proteins, lipids, and other nitrogen (N) metabolites. In this sense, saline stress produces a decrement in the quality and quantity of crop production around the world due to an osmotic and ionic imbalance that alters the N metabolism. The objective of this work is to verify if the genotypic variability and a better nitrogen metabolism regulation improve tolerance to saline stress in tomato plants. This study was conducted with (Grand Brix and Marmande RAF) two tomato commercial genotypes (Solanum lycopersicum L). N forms, N metabolism, N use efficiency (NUE) parameters and amino acid profile were analyzed. A greater GS/GOGAT cycle enzyme activity could promote a better N integration in the plant, besides it promotes the generation of osmoprotective amino acids such as proline and improves the salt stress tolerance. A more effective N metabolism regulation indicates more salt tolerance. Our results showed a better effective N metabolism regulation by Grand Brix.
Assaying the use of sodium thiosulphate as a biostimulant and its effect on cadmium accumulation and tolerance in Brassica oleracea plantsNavarro León, EloyLópez Moreno, Francisco JavierRíos Ruiz, Juan JoséBlasco León, BegoñaRuiz Sáez, Juan Manuelhttps://hdl.handle.net/10481/897522024-03-04T09:04:07ZAssaying the use of sodium thiosulphate as a biostimulant and its effect on cadmium accumulation and tolerance in Brassica oleracea plants
Navarro León, Eloy; López Moreno, Francisco Javier; Ríos Ruiz, Juan José; Blasco León, Begoña; Ruiz Sáez, Juan Manuel
An optimal uptake of mineral elements is crucial to ensure both crop yield and quality. The use of biostimulants is taking relevance to improve the nutrition of crops. Sulphur (S) is one of the elements with great potential within biostimulants. Furthermore, soil contamination by heavy metals such as cadmium (Cd) has become a serious environmental problem. Different studies have suggested the use of thiosulphate (TS) as a biostimulant and to increase the phytoremediation capacity of plants. Therefore, in the present study, we use a crop plant with high S requirements such as Brassica oleracea, to test whether TS serves as a biostimulant and whether affects Cd accumulation and tolerance. B. oleracea plants were grown with two different TS doses (2 mM and 4 mM), under Cd toxicity, and with the combination of Cd toxicity and both TS doses. Parameters of biomass, mineral elements accumulation, and stress tolerance were analyzed. The results showed that TS reduced biomass of B. oleracea plants. The application of 2 mM TS increased Cd accumulation whereas the 4 mM dose reduced it. On the other hand, TS incremented micronutrient accumulation on plants subjected to Cd toxicity and increased Zn contents. Besides, the application of 2 mM to Cd-stressed plants enhanced photosynthesis performance and reduced oxidative stress. Finally, TS increased the antioxidant capacity of B. oleracea plants. Briefly, although TS can not be used as a biostimulant it could be used for Cd phytoremediation purposes and to enhance Zn accumulation in B. oleracea plants.
Tolerance to cadmium toxicity and phytoremediation potential of three Brassica rapa CAX1a TILLING mutantsNavarro León, EloyRuiz Sáez, Juan ManuelAlbacete, AlfonsoBlasco León, Begoñahttps://hdl.handle.net/10481/897512024-03-04T09:03:52ZTolerance to cadmium toxicity and phytoremediation potential of three Brassica rapa CAX1a TILLING mutants
Navarro León, Eloy; Ruiz Sáez, Juan Manuel; Albacete, Alfonso; Blasco León, Begoña
Cadmium (Cd) is one of the most toxic heavy metals that reduces crop productivity and is a threat to all the food chain including human health. Phytoremediation is an environmentally friendly strategy to clean up soil contaminated with heavy metals. Researchers are selecting new varieties with an enhanced capacity for phytoremediation purposes. Three Brassica rapa mutants for CAX1 transporter were obtained through TILLING. The objective of this work is to evaluate the tolerance of these mutants to Cd toxicity and its potential for Cd phytoremediation. For this, the mutants and the parental R-o-18 were grown under control and Cd toxicity conditions (100 µM CdCl2) and growth, Cd accumulation and physiological parameters were analyzed. The results show that BraA.cax1a mutation provides greater Cd uptake capacity although only BraA.cax1a-12 would be useful for phytoremediation because it registered more than three-fold the Cd content of R-o-18 and presented greater Cd tolerance. This tolerance could be due to the higher Ca and Mg accumulations, the maintaining of photosynthesis performance, the enhanced ROS detoxification and AsA/GSH and TCA cycles, the higher malate, and GA4 concentrations and the lower ethylene levels. Briefly, this study identifies BraA.cax1a-12 as a potential mutant for phytoremediation of Cd contaminated soil and identifies possible physiological elements that contribute to this capacity.
Possible role of HMA4a TILLING mutants of Brassica rapa in cadmium phytoremediation programsNavarro León, EloyOviedo-Silva, JhonnatanRuiz Sáez, Juan ManuelBlasco León, Begoñahttps://hdl.handle.net/10481/897502024-03-04T08:20:11ZPossible role of HMA4a TILLING mutants of Brassica rapa in cadmium phytoremediation programs
Navarro León, Eloy; Oviedo-Silva, Jhonnatan; Ruiz Sáez, Juan Manuel; Blasco León, Begoña
Cadmium (Cd) is a dangerous transition element that causes environmental and health problems due to its high mobility in the soil-plant system. In plants, Cd causes serious alterations in physiological processes, affecting different vital functions such as photosynthesis. Species such as Brassica juncea and Brassica rapa have been selected as suitable plants for phytoremediation purposes due to their ability to tolerate the toxic effect of heavy metals. In order to improve this strategy, techniques of plant mutagenesis such as TILLING (Targeting Induced Local Lessions in Genomes) have been employed. In the present work we studied the role of the HMA4 gene in the tolerance to Cd toxicity (100 μM CdCl2) using a TILLING mutant of B. rapa (BraA.hma4a-3). These mutant plants presented a lower biomass reduction and a higher Cd concentration in leaves. An increase in the GSH / GSSG ratio, in the content of photosynthetic pigments and a reduction of oxidative stress was observed, as well as a better photosynthetic index, confirming that BraA.hma4a-3 plants showed a higher tolerance to Cd. In conclusion, according to the results obtained in this work, BraA.hma4a-3 plants could be used for phytoremediation purposes of Cd contaminated soils.
Assaying the efficiency of sulfate, chelate and zinc nanoparticle fertilizers in green bean grown in alkaline soilPonce Garía, Omar CastorNoperi Mosqueda, Linda CitlalliSoto-Parra, Juan ManuelYáñez-Muñoz, Rosa MaríaPérez-Leal, RamonaNavarro León, EloySánchez, Estebanhttps://hdl.handle.net/10481/897412024-03-04T08:16:59ZAssaying the efficiency of sulfate, chelate and zinc nanoparticle fertilizers in green bean grown in alkaline soil
Ponce Garía, Omar Castor; Noperi Mosqueda, Linda Citlalli; Soto-Parra, Juan Manuel; Yáñez-Muñoz, Rosa María; Pérez-Leal, Ramona; Navarro León, Eloy; Sánchez, Esteban
The common bean (Phaseolus vulgaris L.) is one of the most important grain legumes for human diets worldwide due to its nutritional attributes. The application of large amounts of fertilizers is a current problem in agriculture because it could be harmful to the soil and reduce micronutrient availability including zinc (Zn). Zn is an essential element for crop production and quality and also in human diets. Zn deficiency in plants causes biomass reduction, interveinal chlorosis, necrotic spots, browning, small leaves, and growth retardation, and in humans increases the incidence of several diseases. Zn deficiency is common in alkaline soils. Therefore, this study aims to assess the efficiency of ZnSO4, a Zn chelate (DTPA-Zn), and Zn nanoparticles (NfsOZn) as fertilizers in P. vulgaris plants grown in an alkaline soil. To that end, P. vulgaris plants were supplied with the three fertilizers and grown in an experimental greenhouse. Parameters related biomass, NR activity, photosynthetic pigments, and Zn accumulation were analyzed. The three Zn fertilizers, especially DTPA-Zn and NfsOZn, were effective to increase the analyzed parameters. DTPA-Zn was the Zn source that most improved the bean yield and the ZnUpE, whereas NfsOZn produced a greater increase in NR activity, photosynthetic pigments, and ZnUtE. Besides, DTPA-Zn and NfsOZn considerably increased the Zn content in beans. Overall, the application of 50 ppm NfsOZn is the optimum Zn fertilizer to ensure a good yield, quality, and Zn content in P. vulgaris grown in an alkaline soil.