APh, vol. 50(2)https://hdl.handle.net/10481/262582024-03-29T23:04:26Z2024-03-29T23:04:26ZHook effect in radioligand assay for Anti Glutamic Acid Decarboxylase (Anti-GAD65). Influence of temperature and physicochemical interpretationPeris-Conejero, T.Olivares-Pallerols, R.García-Ruiz, H.Moreno-Frigols, J.I.https://hdl.handle.net/10481/274692021-06-15T16:40:56ZHook effect in radioligand assay for Anti Glutamic Acid Decarboxylase (Anti-GAD65). Influence of temperature and physicochemical interpretation
Peris-Conejero, T.; Olivares-Pallerols, R.; García-Ruiz, H.; Moreno-Frigols, J.I.
Background: Radioligand assay is one of the principal methods used for analytical
determination of the Anti-GAD65 concentration. We studied the influence of temperature on
the calibration curves obtained by such a method
Matherial and Methods: We used a commercially available RIA kit for Anti-GAD65 and a
gamma counter. Data was analyzed using Statistica software.
Results and Discusion: Activities bound to the antibody increase with temperature. There
was a decrease in activity for high concentrations attributable to the "hook effect". We propose a simple physicochemical model that justifies satisfactorily the results.; Objetivo: El análisis por radioligando es uno de los métodos principales utilizados en la
determinación analítica del Anti-GAD65. Se ha estudiado la influencia de la temperatura
sobre las gráficas de calibración obtenidas por dicha técnica.
Material y Métodos: Usamos un kit comercial para Anti-GAD65 y un contador gamma. Los
resultados son analizados mediante el programa Statistica.
Resultados y Discusión: Las actividades ligadas al anticuerpo aumentan con la temperatura.
Se observa una disminución de la actividad para altas concentraciones atribuible al llamado “efecto anzuelo”. Se propone un sencillo modelo fisicoquímico que justifica
satisfactoriamente los resultados
Development and validation of enzymatic method for the determination of alcohol in immunoglobulin and albuminCarrillo-Cabrera, D.González-Farah, A.Rodríguez-Bernal, J.Suárez-Pérez, Y.Fernández-Cervera, M.Veliz Rivera, J.https://hdl.handle.net/10481/274682021-06-15T16:40:55ZDevelopment and validation of enzymatic method for the determination of alcohol in immunoglobulin and albumin
Carrillo-Cabrera, D.; González-Farah, A.; Rodríguez-Bernal, J.; Suárez-Pérez, Y.; Fernández-Cervera, M.; Veliz Rivera, J.
Se desarrolló y validó un método enzimático para la cuantificación del contenido de alcohol en los IFAs de Inmunoglobulina y Albúmina. En ambos casos el procedimiento analítico
resultó lineal, exacto, preciso y específico para el control de calidad. Se demostró que el
método fue lineal en el rango de 6.0 a 19.0 mg de alcohol/g de proteína para la albúmina y de
9.2 a 27.6 mg de alcohol/g de proteína para la inmunoglobulina, respectivamente. El método sugerido se aplicó con éxito en la determinación del contenido de alcohol como impureza en lotes industriales.; An enzymatic method for the quantification of alcohol content in immunoglobulin and
albumin was developed and validated. In both materials, the analytical procedure was linear,
accurate, precise and specific. The method was linear in the range from 6.0 to 19.0 mg of
alcohol/g of protein to albumin and to immunoglobulin from 9.2 to 27.6 mg of alcohol/g of
protein, respectively. The proposed method was applied successfully in industrial batches for the determination of the alcohol content as impurity.
Formulation and evaluation of sustained release microspheres of rosin containing aceclofenacLakshmana Prabu, S.Shirwaikar, A.A.Shirwaikar, A.Kumari, A.https://hdl.handle.net/10481/274672021-06-15T16:40:54ZFormulation and evaluation of sustained release microspheres of rosin containing aceclofenac
Lakshmana Prabu, S.; Shirwaikar, A.A.; Shirwaikar, A.; Kumari, A.
Aceclofenac was microencapsulated using rosin by o/w emulsion solvent evaporation
technique. The effect of three formulation variables including the drug:polymer ratio,
emulsifier (polyvinyl alcohol) concentration and organic solvent (dichloromethane) volume
were examined. The prepared batches were characterized for microspheres particle size
distribution, encapsulation efficiency and in vitro release behavior. The study reveals that
drug:polymer ratio had a considerable effect on the entrapment efficiency, however particle
size distribution of microspheres was more dependent on the volume of dichloromethane and
polyvinyl alcohol concentration rather than on the drug: polymer ratio. Drug, polymer
concentrations were varied to obtain optimum release profile for sustaining the action of the drug.
Drug delivery systems based on poly(e-caprolactone) for cancer treatmentSáez-Fernández, E.Ruiz Martínez, María A.Arias Mediano, José Luishttps://hdl.handle.net/10481/274662021-06-15T16:40:54ZDrug delivery systems based on poly(e-caprolactone) for cancer treatment
Sáez-Fernández, E.; Ruiz Martínez, María A.; Arias Mediano, José Luis
Chemotherapy agents have little or no specificity over cancer cells, resulting in low therapeutic concentrations at the tumor site (a consequence of a broad systemic distribution),
and severe side effects. With the aim of avoiding cancer therapy failure, several approaches
such as design of new anticancer drugs, chemical engineering of conventional drugs and
development of drug delivery systems have been proposed. The objective is to enhance drug
localization at the tumor region (by controlling its biodistribution profile) and, therefore, to
increase the anti-tumor efficacy (even in multi-drug resistant tumors), while reducing
systemic side effects. One of the most promising approaches to the problem is the
development of drug nanocarriers based on the polymer poly(e-caprolactone). In this review
we will focus our attention on these polymeric colloids, particularly on the most significant
characteristics and formulation procedures, and on their use as nanoplatforms for the delivery
of chemotherapy agents to the tumor site. Furthermore, the most recent in vitro and in vivo investigations on the subject are extensively reviewed.
Adjudication of new community pharmacies: comparison of ranking criteriaRíos, L.M.Garrigues, T.M.Martín, A.Muelas, J.A.https://hdl.handle.net/10481/274642021-06-15T16:40:57ZAdjudication of new community pharmacies: comparison of ranking criteria
Ríos, L.M.; Garrigues, T.M.; Martín, A.; Muelas, J.A.
This article studies the differences between the models of candidate classification for the adjudication of new pharmacies in the different Spanish self-governing regions. The objective of this study is to analyze the profile of the selected professional. Generally, the ranking takes into account the professional activity as pharmacist, the pre-graduate formation as well as the postgraduate, with different weights. Some of the 15 autonomies include as merits the cooficial languages, an optional written test and special situations as unemployment.
In general, the criteria try to find out the best profile of pharmacist based on the professional experience and the postgraduate formation.