Ars Pharma 2010, Vol. 51https://hdl.handle.net/10481/246642024-03-29T16:00:28Z2024-03-29T16:00:28ZPreparation, Characterization and In Vitro Evaluation of Aceclofenac Solid DispersionsDua, KamalPabreja, KavitaRamana, M. V.https://hdl.handle.net/10481/602162021-06-15T16:41:10ZPreparation, Characterization and In Vitro Evaluation of Aceclofenac Solid Dispersions
Dua, Kamal; Pabreja, Kavita; Ramana, M. V.
The objective of the present investigation was to study the effect of various water soluble carriers like urea, mannitol, PVP and PVP/VA-64 on in vitro dissolution of aceclofenac from solid dispersions. Aceclofenac binary solid dispersions (SD) with different drug loadings were prepared using the melting or fusion method. In vitro dissolution of pure drug, physical mixtures and solid dispersions were carried out. Solid dispersion of aceclofenac with all four carriers (urea, mannitol, PVP and PVP/VA-64) showed considerable increase in the dissolution rate in comparison with physical mixture and pure drug in 0.1 N HCl, pH1.2 and phosphate buffer, pH, 7.4. Solid dispersions containing PVP showed maximum dissolution rate in comparison to formulation containing urea, mannitol and PVP/VA-64. Amorphous nature of the drug in solid dispersion was confirmed by scanning electron microscopy and a decrease in enthalpy of drug melting in solid dispersion compared to the pure drug. FT-IR spectroscopy and differential scanning calorimetry studies indicated no interaction between aceclofenac and carriers in solid dispersions in solid state. Dissolution enhancement was attributed to decreased crystallinity of the drug and to the wetting, eutectic formation and solubilizing effect of the carrier from the solid dispersions of aceclofenac. In conclusion, dissolution of aceclofenac can be enhanced by the use of various hydrophilic carriers like urea, mannitol, PVP and PVP/VA-64.
Evaluación de ratas Sprague Dawley como biomodelo para detectar daño en el ADN de leucocitos de sangre periférica y células hepáticas, mediante el ensayo CometArencibia Arrebola, D. F.Rosario Fernández, L. A.https://hdl.handle.net/10481/602152021-06-15T16:41:13ZEvaluación de ratas Sprague Dawley como biomodelo para detectar daño en el ADN de leucocitos de sangre periférica y células hepáticas, mediante el ensayo Comet
Arencibia Arrebola, D. F.; Rosario Fernández, L. A.
Las rupturas de cadena simple y la formación de sitios lábiles al álcali en el ADN han sido parámetros ampliamente utilizados para la detección de genotoxicidad y han sido demostradas sus implicaciones en enfermedades degenerativas, el cáncer, y recientemente están vinculadas al estrés oxidativo. Este artículo tuvo como objetivo realizar una comparación en cuanto a la frecuencia espontánea (basal) e inducida con ciclofosfamida, de las rupturas de cadena simple y la formación sitios lábiles al álcalis en el ADN de leucocitos de sangre periférica y células hepáticas en ratas Sprague Dawley de ambos sexos, mediante el ensayo Comet. Se administraron 10 ratas/sexo/grupo durante 14 días, utilizando un grupo control negativo (no administrado), dos controles con sustancias vehículo y un control positivo administrado con ciclofosfamida 50 mg/kg por vía intraperitoneal. Pasado este tiempo se realizo la electroforesis alcalina de células inviduales en gel de leucocitos de sangre periférica y de las células hepáticas, para demostrar el posible daño en el ADN. Se concluyó que esta línea de rata constituye un buen modelo experimental in vivo para demostrar el daño primario en el ADN dado la baja frecuencia espontánea de los indicadores analizados. Además bajo nuestras condiciones experimentales resulta más factible la utilidad de este biomodelo en las determinaciones de daño en leucocitos de sangre periférica en ambos sexos.; The induction of strand breaks (SB) or alkali-labile sites formation on DNA have been parameters broadly used for the genotoxicity detection and their implications have been demonstrated in degenerative illnesses, the cancer, and recently linked to the oxidative stress. This article had as objective to carry out a comparison to the spontaneous basal frequency and induced with cyclophosphamide, the induction of strand breaks or alkali-labile sites formation on DNA of leukocytes of peripheral blood and hepatic cells of Sprague Dawley rats of both sexes, by means of the Comet Assay. We administered 10 rats/sex/group for 14 days, using a negative control group (not administered), two substance-vehicle controls and positive control cyclophosphamide administered with 50 mg/kg, intraperitoneal route. The last time of administered was performed the alkaline electrophoresis them gel individual cells from leukocytes of peripheral blood and hepatic cells to demonstrate the possible damage to the DNA. These rat line constitute a good in vivo experimental model to demonstrate the primary damage in the DNA given the low spontaneous frequency of the analyzed indicators. Also under our experimental conditions it is more feasible the biomodels utility in the determinations of damage in leukocytes of peripheral blood in both sexes.
Farmacocinética del Busulfán: Absorción gastrointestinal e influencia del DMSONavarro Moreno, M. A.Moreno Gálvez, A.Moreno Gálvez, E.https://hdl.handle.net/10481/602082021-06-15T16:41:13ZFarmacocinética del Busulfán: Absorción gastrointestinal e influencia del DMSO
Navarro Moreno, M. A.; Moreno Gálvez, A.; Moreno Gálvez, E.
Se ha estudiado la farmacocinética de la absorción gastrointestinal del busulfán en dos grupos de ratas, tras su administración por vía oral. La dosis influye en el valor de la constante de absorción (Ka) y en la concentración máxima normalizada (Cmax), presentando ambos parámetros una disminución al aumentar la dosis de 0,5 a 5 mg. El empleo de un modelo aparente monocompartimental con absorción de primer orden permite una adecuada descripción del perfil de niveles plasmáticos del antineoplásico. El dimetilsulfóxido, utilizado como disolvente del busulfán, no interfiere en los parámetros farmacocinéticos orales del mismo.; We have studied the pharmacokinetics of gastrointestinal absorption of busulfan in two groups of rats, after oral administration. The dose effect on the value of the absorption constant (Ka) and the normalized maximum concentration (Cmax), presenting both parameters decreased with increasing dose from 0.5 to 5 mg. Using a model with monocompartimental apparent first order absorption allows an adequate description of the profile of plasma levels of antineoplastic. The dimethylsulfoxide used as busulfan solvent, does not interfere with oral pharmacokinetic parameters of it.
Interaction of palmitic acid with losartan potassium at the binding sites of bovine serum albuminFerdosi Kabir, A.Nazim Uddin, K.Nazmus Sadat, A.F.M.Mahboob, HossainAbdul Mazid, M.https://hdl.handle.net/10481/601832021-06-15T16:41:11ZInteraction of palmitic acid with losartan potassium at the binding sites of bovine serum albumin
Ferdosi Kabir, A.; Nazim Uddin, K.; Nazmus Sadat, A.F.M.; Mahboob, Hossain; Abdul Mazid, M.
The binding of losartan potassium, an angiotensin II receptor antagonist, to bovine serum albumin was studied by equilibrium dialysis method (ED) in presence or absence of palmitic acid. The study was carried out using ranitidine and diazepam as site-1 and site-2 specific probe, respectively. Different analysis of binding of losartan to bovine serum albumin suggested two sets of association constants: high affinity association constant (k1 = 11.2 x 105 M-1) with low capacity (n1 = 2) and low affinity association (k2 = 2. 63 x 105 M-1) constant with high capacity (n2 = 10) at pH 7.4 and 27°C. During concurrent administration of palmitic acid and losartan potassium in presence or absence of ranitidine or diazepam, it was that found that palmitic acid causes the release of losartan potassium from its binding site on BSA resulting reduced binding of losartan potassium to BSA. The increment in free fraction of losartan potassium was from 13.1% to 47.2 % upon the addition of increased concentration of only palmitic acid at a concentration of 0 x 10-5 M to 16 x 10-5 M. In presence of ranitidine or diazepam as site specific probes, palmitic acid further increases the free fraction of losartan potassium were from 22.8% to 53.4% and 35.3 to 65.5%, respectively. This data provided the evidence of interaction of higher concentration of palmitic acid at the binding sites on BSA changing the pharmacokinetics properties of losartan potassium.
Consumo de suplementos dietarios. Mirando una comunidad universitariaCabral Pérez, MatíasBirri, M.Agnese, Alicia Marielhttps://hdl.handle.net/10481/601792021-06-15T16:41:11ZConsumo de suplementos dietarios. Mirando una comunidad universitaria
Cabral Pérez, Matías; Birri, M.; Agnese, Alicia Mariel
Introducción: Con el objetivo de realizar un estudio epidemiológico que permitiera conocer el consumo de suplementos dietarios (SD) (especialmente de los que incluyeran hierbas) en una comunidad universitaria de la ciudad de Córdoba, se realizó un estudio de Corte. Es importante conocer la proporción de consumo de SD de esta población, habida cuenta de la muy grande y variada oferta de estos productos en el mercado. Materiales y Métodos: La recolección de datos se hizo a partir de dos encuestas, una a estudiantes universitarios (EU) y otra a agentes universitarios o sus familiares (AUF). Resultados: Los resultados muestran que no hubo diferencias significativas en el consumo entre los EU (10,60% [IC 95 %: 8,44 – 12,76]) y los AUF (14,71% [IC 95 %: 12,31 – 17,11] (X2= 0,38, gl= 1, p > 0,05). Discusión: Del análisis de los datos aportados en la encuesta se desprende entre otros, que la razón principal por la que se consumen SD es con fines terapéuticos, lo que se contradice con el concepto de SD. Los resultados sugieren que éstos están inadecuadamente considerados como alimentos y que deberían revisarse las normativas vigentes que los rigen.; Introduction: With the objective of develop an epidemiologic study that allow us to know the dietary supplement (DS) consume (especially of those that contain herbs) in a university community of Córdoba city, a transversal study was developed. It is important to know which is the DS proportion of consume in this community due to the very big and diverse offer of these products present in the market. Material and Methods: Data were collected by using two surveys applied on university students (EU), and the other one, among university agents or their relatives (AUF), respectively. Results: There was not significant difference in the consume between the EU (10,60% [IC 95 %: 8,44 – 12,76]), and the AUF (14,71% [IC 95 %: 12,31 – 17,11]) (X2= 0,38; df= 1, p > 0,05)). Discussion: From the data afforded by the surveys it can be inferred among others, that the main reason for DS consume are therapeutic purposes. Such asseveration contradicts the DS concept. This suggests that DS are inadequately considered as food and that a revision of the actual regulations should be done.
os autores agradecen a las autoridades de la Obra Social Universitaria DASPU como así también a las de las Facultad de Arquitectura, Urbanismo y Diseño; Facultad de Ciencias Exactas, Físicas y Naturales; Facultad de Ciencias Económicas; Facultad de Ciencias Agropecuarias; Escuela de Trabajo Social y Facultad de Odontología, por permitir la realización del presente trabajo.