Grupo: Fotoquímica y Fotobiología (FQM 247)https://hdl.handle.net/10481/146512024-03-28T17:35:18Z2024-03-28T17:35:18ZPhotosensitizing properties and subcellular localisation of 3,4‑dihydro‑β‑carbolines harmaline and harmalolDenofrio, M. PaulaParedes Martínez, José ManuelYañuk, Juan G.Girón González, María DoloresSalto González, RafaelTalavera Rodríguez, Eva MaríaCrovetto González, LuisCabrerizo, Franco M.https://hdl.handle.net/10481/887312024-02-08T12:03:51ZPhotosensitizing properties and subcellular localisation of 3,4‑dihydro‑β‑carbolines harmaline and harmalol
Denofrio, M. Paula; Paredes Martínez, José Manuel; Yañuk, Juan G.; Girón González, María Dolores; Salto González, Rafael; Talavera Rodríguez, Eva María; Crovetto González, Luis; Cabrerizo, Franco M.
Harmaline (1) and harmalol (2) represent two 3,4-dihydro-β-carboline (DHβCs) most frequently reported in a vast number
of living systems. Fundamental aspects including the photosensitizing properties, cellular uptake, as well as the cyto- and
phototoxicity of 1 and 2 were investigated herein. The molecular basis underlying the investigated processes are elucidated.
Data reveal that both alkaloids show a distinctive pattern of extracellular DNA photodamage. Compound 1 induces a DNA
photodamage profile dominated by oxidised purines and sites of base loss (AP sites), whereas 2 mostly induces single-strand
breaks (SSBs) in addition to a small extent of purine oxidative damage. In both cases, DNA oxidative damage would occur
through type I mechanism. In addition, a concerted hydrolytic attack is suggested as an extra mechanism accounting for the
SSBs formation photoinduced by 2. Subcellular internalisation, cyto- and phototoxicity of 1 and 2 and the corresponding
full-aromatic derivatives harmine (3) and harmol (4) also showed quite distinctive patterns in a structure-dependent manner.
These results are discussed in the framework of the potential biological, biomedical and/or pharmacological roles reported
for these alkaloids.
Efficient acetate sensor in biological media based on a selective Excited State Proton Transfer (ESPT) reactionPuente Muñoz, VirginiaParedes Martínez, José ManuelResa Acosta, SandraOrtuño Guzmán, Ana MaríaTalavera Rodríguez, Eva MaríaMiguel Álvarez, DeliaCuerva Carvajal, Juan ManuelCrovetto González, Luishttps://hdl.handle.net/10481/867232024-01-11T13:08:46ZEfficient acetate sensor in biological media based on a selective Excited State Proton Transfer (ESPT) reaction
Puente Muñoz, Virginia; Paredes Martínez, José Manuel; Resa Acosta, Sandra; Ortuño Guzmán, Ana María; Talavera Rodríguez, Eva María; Miguel Álvarez, Delia; Cuerva Carvajal, Juan Manuel; Crovetto González, Luis
We have synthesized a new fluoride−containing xanthenic dye able to dynamically and
quantitatively detect acetate anion, a biologically relevant analyte, in water. We studied
deeply the photophysical properties of the compound and verified its use as an acetate
probe in synthetic serum.
Exchangeable Self-Assembled Lanthanide Antennas for PLIM MicroscopyRuiz Arias, ÁlvaroFueyo-Gonzalez, FranciscoIzquierdo-Garcia, CarolinaNavarro, AmparoGutierrez-Rodriguez, MartaHerranz, RosarioBurgio, ChiaraReinoso, AntonioCuerva Carvajal, Juan ManuelOrte Gutiérrez, ÁngelGonzález Vera, Juan Antoniohttps://hdl.handle.net/10481/860022023-12-04T07:51:51ZExchangeable Self-Assembled Lanthanide Antennas for PLIM Microscopy
Ruiz Arias, Álvaro; Fueyo-Gonzalez, Francisco; Izquierdo-Garcia, Carolina; Navarro, Amparo; Gutierrez-Rodriguez, Marta; Herranz, Rosario; Burgio, Chiara; Reinoso, Antonio; Cuerva Carvajal, Juan Manuel; Orte Gutiérrez, Ángel; González Vera, Juan Antonio
Lanthanides have unique photoluminescence (PL) emission properties, including very long PL life- times. This makes them ideal for biological imaging applications, especially using PL lifetime imaging micro- scopy (PLIM). PLIM is an inherently multidimensional technique with exceptional advantages for quantitative biological imaging. Unfortunately, due to the required prolonged acquisitions times, photobleaching of lantha- nide PL emission currently constitutes one of the main drawbacks of PLIM. In this study, we report a small aqueous-soluble, lanthanide antenna, 8-methoxy-2-oxo- 1,2,4,5-tetrahydrocyclopenta[de]quinoline-3-phosphonic acid, PAnt, specifically designed to dynamically interact with lanthanide ions, serving as exchangeable dye aimed at mitigating photobleaching in PLIM microscopy in cellulo. Thus, self-assembled lanthanide complexes that may be photobleached during image acquisition are continuously replenished by intact lanthanide antennas from a large reservoir. Remarkably, our self-assembled lanthanide complex clearly demonstrated a significant reduction of PL photobleaching when compared to well- established lanthanide cryptates, used for bioimaging. This concept of exchangeable lanthanide antennas opens new possibilities for quantitative PLIM bioimaging.
This is the published manuscript version of the following manuscript:
Exchangeable Self-Assembled Lanthanide Antennas for PLIM Microscopy, Angew. Chem. Int. Ed. 2023, e202314595 doi: https://doi.org/10.1002/anie.202314595
This article may be used for non-commercial purposes in accordance with Wiley Sharing Policies.
Supplementary Materials accompanying this article can be found on-line at the publisher’s site.
Video de la síntesis de AgNRs mediante poliolesGonzález García, María del Carmenhttps://hdl.handle.net/10481/758002022-07-04T07:11:21ZVideo de la síntesis de AgNRs mediante polioles
González García, María del Carmen
Video de la síntesis de AgNRs mediante polioles
Video of polyol synthesis of AgNRs
Interacción de AgNRs en disolución con un láser pulsado de 375 nmGonzález García, María del Carmenhttps://hdl.handle.net/10481/757992022-07-04T07:10:04ZInteracción de AgNRs en disolución con un láser pulsado de 375 nm
González García, María del Carmen
Video y simulación de la interacción de las AgNRs con un láser pulsado de 375 nm.
Video and simulation of the interaction between AgNRs and a pulsed laser of 375 nm