@misc{10481/55571,
year = {2018},
month = {7},
url = {http://hdl.handle.net/10481/55571},
abstract = {Hydrolysates of food protein sources have immunomodulatory effects, which are of interest
for use as functional foods. In this study, we have characterized the immune regulatory effect
on rat splenocytes, macrophages and T lymphocytes of Ulva spp. hydrolysates and their peptide
fractions with or without in vitro gastrointestinal digestion and/or ultrafiltration. IL-10 was induced
in almost all conditions and cell types obtained from wild type animals. The induction was in
general increased by ultrafiltration and in vitro gastrointestinal digestion. TNF was also induced in
basal conditions. In turn, TNF and IFN-
 production was attenuated by the hydrolysate products
in lipopolysaccharide or concanavalin A immune stimulated cells. Inhibitors for the activation
of NF B, MAPK p38 and JNK inhibited IL-10 induction in rat splenocytes. The response was
dramatically attenuated in TLR4-/- cells, and only modestly in TLR2-/- cells. Food peptides
from Ulva spp. genus exert anti-inflammatory effects in immune cells mediated by TLR4 and NF B.
Similarity with the immunomodulatory profile of protein hydrolysates from other sources suggests a
common mechanism.},
organization = {This work was supported by funds from the Ministry of Economy and Competitivity, partly with
Fondo Europeo de Desarrollo Regional FEDER funds [SAF2017-88457-R, AGL2017-85270-R, BFU2014-57736-P,
AGL2014-58883-R] and by Junta de Andalucía [CTS235, CTS164]. C.H.-C. and R.G.-B. were supported by
the University of Granada (Contrato Puente Program—Plan Propio) and the Ministry of Education [Spain],
respectively. CIBERehd is funded by Instituto de Salud Carlos III.},
publisher = {MDPI},
keywords = {Bioactive peptides},
keywords = {Green seaweeds},
keywords = {Interleukin 10},
keywords = {TLR2},
keywords = {TLR4},
keywords = {NFkB},
title = {Green Alga Ulva spp. Hydrolysates and Their Peptide Fractions Regulate Cytokine Production in Splenic Macrophages and Lymphocytes Involving the TLR4-NFkB/MAPK Pathways},
author = {Cian, Raúl E. and Hernández Chirlaque, Cristina and Gámez Belmonte, María de los Reyes and Drago, Silvina R. and Sánchez De Medina López-Huertas, Fermín and Martínez Augustín, María Olga},
}