@misc{10481/32021,
year = {2014},
url = {http://hdl.handle.net/10481/32021},
abstract = {Supernumerary (B) chromosomes have been shown to contain a wide variety of repetitive sequences. For this reason, fluorescent in situ hybridisation (FISH) is a useful tool for ascertaining the origin of these genomic elements, especially when combined with painting from microdissected B chromosomes. In order to investigate the origin of B chromosomes in the fish species Astyanax paranae, these two approaches were used along with PCR amplification of specific DNA sequences obtained from the B chromosomes and its comparison with those residing in the A chromosomes. Remarkably, chromosome painting with the one-arm metacentric B chromosome probe showed hybridization signals on entire B chromosome, while FISH mapping revealed the presence of H1 histone and 18S rDNA genes symmetrically placed in both arms of the B chromosome. These results support the hypothesis that the B chromosome of A. paranae is an isochromosome. Additionally, the chromosome pairs Nos. 2 or 23 are considered the possible B chromosome ancestors since both contain syntenic H1 and 18S rRNA sequences. The analysis of DNA sequence fragments of the histone and rRNA genes obtained from the microdissected B chromosomes showed high similarity with those obtained from 0B individuals, which supports the intraspecific origin of B chromosomes in A. paranae. Finally, the population hereby analysed showed a female-biased B chromosome presence suggesting that B chromosomes in this species could influence sex determinism.},
organization = {This research was funded by grants from the State of São Paulo Research Foundation (FAPESP) to DMZAS (2011/16825-3) and CO (2010/17009-2), grants from National Council for Research and Development (CNPq) to FF and by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).},
publisher = {Public Library of Science (PLOS)},
keywords = {Chromosome mapping},
keywords = {Chromosomes},
keywords = {DNA},
keywords = {DNA sequence analysis},
keywords = {Fungal genomics},
keywords = {Histones},
keywords = {Sex chromosomes},
title = {Delimiting the Origin of a B Chromosome by FISH Mapping, Chromosome Painting and DNA Sequence Analysis in Astyanax paranae (Teleostei, Characiformes)},
doi = {10.1371/journal.pone.0094896},
author = {Silva, Duílio M. Z. de A. and Pansonato-Alves, José Carlos and Utsunomia, Ricardo and Araya-Jaime, Cristian and Ruiz-Ruano, Francisco J. and Natal Daniel, Sandro and Teruo Hashimoto, Diogo and Oliveira, Cláudio and Martínez Camacho, Juan Pedro and Porto-Foresti, Fábio and Foresti, Fausto},
}