@misc{10481/30964,
year = {2009},
url = {http://hdl.handle.net/10481/30964},
abstract = {Cold ischemia time especially impacts on outcomes of expanded-criteria donor (ECD) transplantation. Ischemia-reperfusion (IR) injury produces excessive poly[ADP-Ribose] Polymerase-1 (PARP-1) activation. The present study explored the hypothesis that increased tubular expression of PARP-1 contributes to delayed renal function in suboptimal ECD kidney allografts and in non-ECD allografts that develop posttransplant acute tubular necrosis (ATN).

[Materials and Methods]

Nuclear PARP-1 immunohistochemical expression was studied in 326 paraffin-embedded renal allograft biopsies (193 with different degrees of ATN and 133 controls) and in murine Parp-1 knockout model of IR injury.

[Results]

PARP-1 expression showed a significant relationship with cold ischemia time (r coefficient = 0.603), time to effective diuresis (r = 0.770), serum creatinine levels at biopsy (r = 0.649), and degree of ATN (r = 0.810) (p = 0.001, Pearson test). In the murine IR model, western blot showed an increase in PARP-1 that was blocked by Parp-1 inhibitor. Immunohistochemical study of PARP-1 in kidney allograft biopsies would allow early detection of possible delayed renal function, and the administration of PARP-1 inhibitors may offer a therapeutic option to reduce damage from IR in donor kidneys by preventing or minimizing ATN. In summary, these results suggest a pivotal role for PARP-1 in the ATN of renal transplantation. We propose the immunohistochemical assessment of PARP-1 in kidney allograft biopsies for early detection of a possible delayed renal function.},
organization = {This work was funded by the Fondo de Investigaciones Sanitarias (Grants PI021505, and PI051197).},
publisher = {Public Library of Science (PLOS)},
keywords = {Biopsy},
keywords = {Creatinine},
keywords = {Kidneys},
keywords = {Renal analysis},
keywords = {Renal ischemia},
keywords = {Renal system},
keywords = {Renal transplantation},
keywords = {Transplantation immunology},
title = {Poly[ADP-Ribose] Polymerase-1 Expression Is Related To Cold Ischemia, Acute Tubular Necrosis, and Delayed Renal Function In Kidney Transplantation},
author = {O'Valle Ravassa, Francisco Javier and García Del Moral Garrido, Raimundo and Benítez Ortúzar, María Del Carmen and Martín Oliva, David and Gómez-Morales, Mercedes and Aguilar Peña, David and Aneiros-Fernández, José and Hernández-Cortés, Pedro and Osuna Ortega, Antonio and Moreso, Francesc and Serón, Daniel and Oliver Pozo, Francisco Javier and García Del Moral Garrido, Raimundo},
}